Author(s): Shilaja Yougender Nayini*, Chandra ShakarReddy Nalagoni, Rajkiran Reddy Banala, Sabita S.Raju
Coleus forskohlii (CF) is a well-known medicinally important plant and it has been part of Ayurveda for decades. Forskolin, a diterpene and active constituent of Coleus forskohliiroot extract (CFRE). The CFRE has been used for treating various infections and chronic diseases. But the CFRE has never been used as a pesticide. Exorista bombycis(Uzi fly) is a common pest found to infest Bombyx mori(Silk worm) and cause severe loss to sericulture farmers or community. Uzi fly targets the fifth instar larvae of silk worm to lay its eggs and for the propagation of its species. CFRE and Forskolin were evaluated for its anti-Uzi fly properties and the Uzi fly maggots, Uzi fly pupae were treated with different concentrations of CFRE and Forskolin were used i.e. 0.5, 1.0, 1.5, 2.0 μg/μL. It was observed that the normal growth and development of Uzi fly were hindered at different stages of its life cycle. The fifth instar larvae of silkworm were treated with 0.5-2.0 μL of CFRE and Forskolin which resulted in oviposition deterrence, reduced egg hatching, aberrations and deformities in pupae and adult (i.e. molting process, ecdysial failure) and adult emergence from pupae. The observations from the present study clearly suggest that CFRE and Forskolin can be an alternative for commercially available pesticides for preventing the propagation of Uzi fly by influencing its growth, development, and metamorphosis of immature stages of Uzi fly. The efficacy of CFRE is slightly better than Forskolin in exerting its anti-Uzi fly properties.