Anatomical, phytochemical andin vitroantimicrobial studies of HyptissuaveolensL. Poit. of family Lamiaceae

Abstract

Author(s): TuluguMadhuri, B.Sujatha, Seetha Lakshmi B

The present study deals with the anatomical, phytochemical and antimicrobial investigations on the Lamiaceae member ofHyptis suaveolens(L). Poit, collected from the district Visakhapatnam, Andhra Pradesh, India.H. suaveolensL. Poit. the commonest plant in India.It is an undershrub. Stem quadrangular, hairy, leaves aromatic, ovate, tomentose, pale green, turn into the purplish-green at the flowering stage. Anatomical characters observed to be upper and lower epidermis layers of the leaves were covered by multicellular hairs. Vascular bundles were well developed with secondary growth with broad vessels. An attempt has been made to carry out screening phytochemical constituents and antimicrobial ability of various extract ofH. suaveolens(L.) poit. Shade dried aerial parts were used. The qualitative analysis confirmed the presence of alkaloids, terpenoids, and phenols in leaves. Antimicrobial activity was screened by the agar well diffusion method. Activities of hexane, chloroform and methanol extracts ofH. suaveolens(L.) poit were tested againstBacillus subtilis MTCC 441, Enterococcus faecalis MTCC 439, Staphylococcus aureus MTCC 737, Escherichia coli MTCC 443, Proteus vulgaris MTCC 426, Pseudomonas aeruginosa MTCC 1688, Candida albicans MTCC 227, Epidermophyton floccosumMTCC 613, and Trichophyton mentagrophytes MTCC 7687. The antimicrobial activity potential was observed by measuring the width of the inhibitory zones. Hexane and chloroform extract showed mild to moderate inhibitory activity against organisms tested. The methanolic extract showed the highest inhibitory activity against tested microflora. This scientific investigation may be utilized to develop bio fungicide from this plant to control skin diseases in humans.

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