Author(s): Sunita Panchawat
Chemo-profiling of herbal drugs represent a comprehensive qualitative approach for the purpose of species authentication, evaluation of quality and ensuring the consistency and stability of drugs and their related products. TLC were performed for preliminary identification of constituent in solvent system Toluene:Chloroform:Acetone (40:25:35 v/v/v), gallic acid was used as standard for phenolic compound. Phenolic compound was estimated in hydro-alcoholic extract ofFicus religiosaLinn. (Stem bark) by high performance thin layer chromatography (HPTLC). Precoated Silica gel 60 F 254 (E. Merk) TLC plates were used as stationary phase and Toluene: Chloroform: Acetone (40:25:35 v/v/v) was used as mobile phase. Detection and quantification were performed by densitometry at λ254 nm. The linear range was 200 ng to 600 ng. This HPTLC method was found to reproducible, accurate and precise.