Inhibition of colon carcinoma cell lines by treatment with crude spine venom from marine catfish Tachysurus dussumieri

Abstract

Author(s): KavithaRaja, SharonSelvaraj , Arulvasu Chinnasamy

Catfishes, comeunder the order Siluriformes, they representas venomous fishes havingvenom glands in that dorsal and pectoral spines that are spearedinto place when threatened. The catfish, Tachysurus dussumierispine venom is unsafe and having neuromuscular blocking activity, envenoms causes instant, local and intense pain, and a variable extent of bleeding. The present study was carried outinthe histological section of spine and effect of spine venom on human colon cancer cell lines (HT 29 and COLO 320). Histology study of spine shows the presence of venom secretory cells. Venom wasextracted from spines of catfish and the biochemical composition such as protein (1.020 mg/ml), lipid (0.955 mg/ml) and carbohydrate (0.313 mg/ml) was estimated. Protein profiles of Native and SDSpage were indicating both high and low molecular weight of proteins. Toxicity on brine shrimp nauplii revealed the LC50 observed at 400 mg/ml. Result of spine venomon Vero cell line showed maximum cell viability up to800 mg/ml. The cell viability on HT 29 and COLO 320 cell lines showedIC50at a concentration of 300 mg/ml and 400 mg/ml,at the time of 24 h incubation. Significantly Propidium iodide staining method wasobserved to viewdamaged nucleus of cells. DNA fragmentation assay has confirmed DNA fragments due to the induction of apoptosis.