Bioactive potential of Pseudomonas alcaliphila isolated from a marine sponge against human pathogens

  • Sathiyamurthy K. Molecular Microbial Pathogenesis Laboratory, Department of Biomedical Science, Bharathidasan University, Tiruchy- 620024, Tamilnadu
  • Bavithra H. Molecular Microbial Pathogenesis Laboratory, Department of Bio-Medical Sciences, Bharathidasan University, Trichy-620024.
Keywords: Marine sponge, Antibacterial activity, Minimum Inhibitory Concentration, GC-MS, Bioactive metabolites, Pseudomonas alcaliphila

Abstract

Metabolite extraction is considered as one of the important steps in metabolomics, the marine metabolite are the new source of the most antimicrobial agents used in both pharmacological and biological applications. In the present study, sponge associated bacterial metabolites was investigated. A total of 20 bacterial strains were isolated from the sponge Haliclona sp., All the strains were screened primarily with cross streaking method against human bacterial pathogens. The potent isolate was chosen based on the good inhibitory activity and metabolite extraction was achieved using chloroform: methanol mixture. The metabolites were then checked for their antimicrobial activity by disk diffusion and also minimum inhibitory concentration was determined. Out of 20 bacterial strains, only one strain selected based on the good inhibitory activity against pathogens and the strain was identified as Pseudomonas alcaliphila based on the biochemical and16S rRNA sequencing. The results revealed that the metabolites exhibited high activity and it was found that Klebsiella pneumoniae was inhibited high with the diameter of 22 mm followed by Salmonella Typhi (15 mm), E.coli (12 mm), and Bacillus subtilis (15 mm). The MIC was observed at 31.25 µg/ml against all pathogens. Results of TLC exhibited the Rf value at 0.86 and the FTIR results revealed the presence of C=o, amide bond, amino acids and methoxy groups. In GC-MS results showed that the metabolites mostly contain fatty acids and alkenes compounds. Thus, this marine active compound was considered as a novel compound for biological applications and may be a potential drug for therapeutic use.

References

Bhatnagar, I.; Kim, S. K. Immense essence of excellence: Marine microbial bioactive compounds. Mar. Drugs, 8(10), 2010, 2673-2701.

Taylor, M.W., Radax, R., Steger, D., Wagner, M. Sponge-Associated Microorganisms: Evolution, Ecology, and Biotechnological Potential. Microbiol, Mol. Biol. Rev. 71, 2007, 295–347.

Lanka Sri Santhi, Prasad Talluri VSSL, Nagendra SY and Radha Krishna. Bioactive Compounds from Marine Sponge Associates: Antibiotics from Bacillus sp. Nat Prod Chem. Res, 2017, 5:4.

Erwin, P.M., Pita, L., López-Legentil, S., Turon, X. Stability of Sponge Associated Bacteria over Large Seasonal Shifts in Temperature and Irradiance. Appl. Environ. Microbiol. 78, 2012, 7358–7368.

Thomas, T. R. A.; Kavlekar, D. P.; LokaBharathi, P. A. Marine Drugs from Sponge-Microbe Association–A Review. Mar. Drugs, 2010, 8(4), 1417-1468.

Lee, Y.; Lee, J.; Lee, H. Microbial symbiosis in marine sponges. J. Microbiol., 2001, 39(4), 254-264.

Blunt, J. W.; Copp, B. R.; Keyzers, R. A.; Munro, M. H.; Prinsep, M. R. Marine natural products. Nat. Prod. Rep., 2012, 29(2), 144222.

Kennedy, J.; Baker, P.; Piper, C.; Cotter, P. D.; Walsh, M.; Mooij, M. J.; Bourke, M. B.; Rea, M. C.; O’Connor, P. M.; Ross, R. P.; Hill, C.; O’Gara, F.; Marchesi, J. R.; Dobson, A. D. W. Isolation and analysis of bacteria with antimicrobial activities from the marine sponge Haliclona simulans collected from Irish waters. Mar Biotechnol., (2009), 11(3), 384-396.

Bergmann, W.; Feeney, R. J. Contributions to the study of marine products. XXXII. The nucleosides of sponges. I. J. Org. Chem., 1951, 16(6), 981-987.

Hentschel, U. Hopke, J., Horn, M., Friedrich, A. B., Wagner, M.; Hacker, J.; Moore, B. S. Molecular evidence for a uniform microbial community in sponges from different oceans. Appl. Environ. Microbiol., 68(9), 2002, 4431-4440

Atta HM, Dabour SM, Desoukey SG. Sparsomycin antibiotic production by Streptomyces sp. AZ-NIOFD1: taxonomy, fermentation, purification and biological activities. Am Eurasian J Agric Environ Sci, 5: 2009,368–377 2.

Isao Yumoto, Koji Yamazaki, Megumi Hishinuma, Yoshinobu Nodasaka, Akio Suemori, Kenji Nakajima, Norio Inoue and Kosei Kawasaki. Pseudomonas alcaliphila sp. a novel facultatively psychrophilic alkaliphile isolated from seawater. International Journal of Systematic and Evolutionary Microbiology, 51, 2001, 349–355.

Imada C. Enzyme inhibitors and other bioactive compounds from marine Actinomycetes. Antonie Van Leeuwenhoek 87:59–63, 2005, doi:10.1007/s10482-004-6544-x.

Koul S, Jyotsana P, Anjali M, Kalia VC. Potential emergence of multi-quorum sensing inhibitor resistant (MQSIR) bacteria. Indian J Microbiol 56:1–18, 2016, doi:10.1007/s12088-0150558-0.

Mukherjee K, Tribedi P, Mukhopadhyay B, Sil AK. Antibacterial activity of long-chain fatty alcohols against Mycobacteria. FEMS Microbiol Lett 338:177–183. 2013. doi: 10.1111/ 1574-6968.12043.

Al-Abd NM, Mohamed ZN, Mansor M, Azhar F, Hasan MS, Kassim M. Antioxidant, antibacterial activity, and phytochemical characterization of Melaleuca cajuputi extract. BMC Complement Altern Med 15:1–13, 2015 doi: 10.1186/s12906-0150914-y.

Naoko T, Akiko S, Miki N, Keisuke M, Kazutoyo E, Hajime H, Yoshihiro I. Antibacterial activity of long-chain fatty alcohols against Staphylococcus aureus. Molecule 12:139–148, 2007, doi: 10.3390/12020139.

Walter V, Syldatk C, Hausmann R. Screening concepts for the isolation of biosurfactant producing microorganisms. Adv Exp Med Biol. 2010; 672:1–13.

Selvakumar D, Arun K, Suguna S, Kumar D, Dhevendaran K. Bioactive potential of Streptomyces against fish and shellfish pathogens. Iran J Microbiol 2010; 2(3):157-64.

Ghribi D, Ellouze-Chaabouni S. Enhancement of Bacillus subtilis lipopeptide biosurfactants production through optimization of medium composition and adequate control of aeration. Biotechnol Res Int. 2011:653654.

Villas-Boas SG, Hojer-Pedersen J, Akesson M, Smedsgaard J, Nielsen. Global metabolite analysis of yeast: evaluation of sample preparation methods. Yeast 22:, 2005, 1155-1169.

Ivancheva S, Nikolova M, Tsvetkova R. Pharmacological activities, and biologically active compounds of Bulgarian medicinal plants. Phytochem Adv Res Filippo Imperato Res Signpost ,2006, 87-103.

Alrheam A, Sheik GB, Al Mutairi NBK, Al Mutairi MBK. The efficacy of newly formula of Alkanna tinctoria oil for treatment of five bacterial species. Int J Adv Res, 3: 2015, 646-654.

Usha Nandhini S, Sangareshwari S, Lata K. Gas chromatography-mass spectrometry analysis of bioactive constituents from the marine Streptomyces. Asian J Pharm Clin Res 8: 2015, 244–246.

Salem MZ, Ali HM, Mansour MM. Fatty acid methyl esters from air-dried wood, barks, and leaves of Brachychiton diversifolius R. Br: antibacterial, antifungal, and antioxidant activities. Bioresources 9: 2014, 3835–3845.

Abd El Razak A, Ward A, Glassey J. Response surface methodology for optimising the culture conditions for eicosapentaenoic acid production by marine bacteria. Journal of Industrial Microbiology and Biotechnology In Press: 1-11. 2013, doi:10. 1007/s10295-013-1238-x.

Gentile G, Bonasera V, Amico C, Giuliano L, Yakimov MM. Shewanella sp. GA-22, a psychrophilic hydrocarbonoclastic Antarctic bacterium producing polyunsaturated fatty acids. J Appl Microbiol 95(5):1124–1133. 2003, doi:10.1046/j.1365-2672.2003.02077.x.

Maheshwari, K., K. Saraswathi, D. Sankari and Arumugam, P. Evaluation of Bioactive chemical constituents by Gas chromatography-Mass spectrometry analysis isolated from Bacillus species. Int.J.Curr.Microbiol.App.Sci. 5(1): 2016, 488-497.

Published
2019-03-03
How to Cite
K., S., & H., B. (2019). Bioactive potential of Pseudomonas alcaliphila isolated from a marine sponge against human pathogens. International Journal of Bio-Pharma Research, 8(3), 2494-2503. https://doi.org/10.21746/ijbpr.2019.8.3.2
Section
Research Article