Isolation and characterization of Arbuscular Mycorrhizal (AM) fungi from the rhizospheric soil of some medicinal plants
The present investigation was carried out to estimate the colony forming units of indigenous fungi and to study the diversity and Arbuscular mycorrhizal (AM) of some medicinal plants. The study was also aimed to estimate and isolate the Arbuscular mycorrhizal (AM) spore population from the rhizospheric soil of some important medicinal plants from the Botanical garden of College campus. All the selected medicinal plants i.e., Mentha sp. Ocimum sanctum, Bryophyllum sp. Adhatoda vasica, and Andrographis paniculata showed a very good diversity of rhizospheric soil fungi. The highest number of Colony forming unit (c.f.u) was estimated in Mentha sp (162/10-1) followed by Bryophyllum sp. (156/10-1), Andrographis paniculata (91/10-1), Adhatoda vasica (72/10-1) and Ocimum sanctum (68/10-1). Arbuscular mycorrhizal (AM) spore population was also estimated in all the medicinal plants. The higest number of AM spores was estimated in Andrographis paniculata (121/50gm-1) followed by Ocimum sanctum (112/50gm-1), Adhatoda vasica (94/50gm-1), Bryophyllum sp.(89/50gm-1) and Mentha sp (75/ 50gm-1). The rhizospheric soil fungal diversity including mycorrhizal fungi (Glomus sp.) also was found to be beneficial for increasing the photosynthetic activity and uptake of phosphorus in medicinal plants. Thus, the use of AM inoculum can increase the yield of medicinal plants along with their secondary metabolites to utilize on a sustainable basis as a traditional and herbal medicine for the human welfare.
Barbhuiya A.R., Sharma G.D., Arunachalam A. and Deb S. “Diversity and conservation of medicinal plants in Barak Valley, Northeast India.” Indian. Journal of Traditional. Knowledge. 8 (2009):169-175.
Barik, Bibhuti Prasad, Tayung, Kumananda, Jagadev, Prema Narayan and Dutta, Sushil Kumar. “Phylogenetic placement of an endophytic fungus Fusarium oxysporum isolated from Acoruscalamus rhizome with antimicrobial activity.” European Journal of Biological Sciences 2.1(2010):8-16.
Brundrett Mark. “Mycorrhizal associations and other means of nutrition of vascular plants: understanding the global diversity of host plants by resolving conflicting information and developing reliable means of diagnosis.” Plant Soil 320 (2009): 7-7.
Copetta A., Lingua G. and Berta G., Effects of three AM fungi on growth, distribution of glandular hairs, and essential oil production in Ocimum basilicum L. var. Genovese, Mycorrhiza 16.(2006):485-494.
Chanda, Dhritiman, Sharma, Gauri, Dutt and Jha, Dhruva Kumar (2014). “Isolation and identification of some Arbuscular Mycorrhiza (AM) fungi for phytoremediation in soil contaminated with paper mill effluent.” International Journal of Current Microbiology and Applied Sciences. 3.6(2014):527-539.
Gaur, Supriya and Kaushik, Purshotam. “Analysis of vesicular arbuscular mycorrhiza associated with medicinal plants in Uttarkhand State of India.” World Applied Science Journal. 14 (2011): 645-653.
Gerdemann, J.W and Nicolson, T.H.”.Spores of mycorrhizal Endogone species extracted from soil by wet sieving and decanting.” Trans. Br. Mycol. Soc. 46.2(1963):235-244.
Gentili, Francesco and Jumpponen, Ari. “Potential and possible uses of bacterial and fungal biofertilizers.” In: Rai MK (ed) Handbook of Microbial biofertilizers, Haworth Press, Technology& Engineering, New York, 2006: 1-28.
Guntilaka, Leslie, AY. Natural Products from Plant-Associated Microorganism: Distribution, structural Diversity, Bioactivity and Implications of their occurrence .Journal of Natural Products.69 (2006):509-526.
Ingle, R.W., Sarika, More, Deshmukh, V.V. and Joshi Milind.” Association of vesicular arbuscular mycorrhizae with medicinal plant.” International Journal of Science and Environment. 2 (2013): 1265-1276.
Johansson, Jonas, F., Paul, Leslie R and Finlay, Roger D. “Microbial interactions in the mycorrhizosphere and their significance for sustainable agriculture”. FEMS Microbial Ecology. 48 (2004):1-13.
Joner,Erik.J.andLeyval,Corinne.”Phytoremediation of organic pollutants using mycorrhizal plants: a new aspect of rhizosphere interactionsAgronomie. 23(5-6) (2003): 495-502.
Karthikeyan B., Joe1 M.M. and Jaleel C.A. “Response of Some Medicinal Plants to Vesicular Arbuscular Mycorrhizal Inoculations.” Journal of Scientific Research. 1(2009): 381-386.
Khan A, G. “Role of soil microbes in the rhizospheres of plants growing on trace metal contaminated soilsnin phytoremediation”. Journal of Trace Elements in Medicine and Biology.18 (2000):355-364.
Koberl, Martina., Schmidt, Ruth., Ramadan, Elshahat, M., Bauer, Rudolf, Berg, Gabriele. “The microbiome of medicinal plants: diversity and importance for plant growth, quality and health.” Frontiers in Mircrobiology.4 (2013):400.
Kumar A, Mangla C, Aggarwal A, Parkash, Vipin.” Arbuscular mycorrhizal fungal dynamics in the rhizospheric soil of five medicinal plants species.” Middle-East Journal of Scientific Research. 6 (2010):281-288.
Mathan, Nisha, C and Rajeshkumar, Sevanan. “Effect of arbuscular mycorrhizal fungi on growth and nutrition of Wedilia chinensis (Osbeck) Merril.” Indian Journal of Science and Technology.,3(2010): 676-678.
Mishra M.K., Shukla M. “Fungal flora of some medicinal plants.” International. Journal of Scientific Research. 3.8(2014):1786-1788.
Radhika K.P. and Rodrigues B.F. “Arbuscular mycorrhizal fungal diversity in some commonly occurring medicinal plants of Western Ghats, Goa region.” Journal of Forest Research. 21(2010): 45-52.
Raja P., “Status of endomycorrhizal (AMF) biofertilizer in the global market.” In: Handbook of Microbial Biofertilizers, edited by MK. Rai, Food products press, (2006):395-416.
Sampathkumar G.N, Prabakaran M. and Rajendra R. “Association of AM- fungi in some medicinal plants and its influence on growth.” In: Organic farming and mycorrhizae in agriculture, I. K. Int. Pub. House Pvt. Ltd. New Delhi India, (2007):101-106.
Sagar A., Kumar R. “Fungal associates of Centella asiatica and Ocimum sanctum.” Journal of Pure and Applied Microbiology.3(2009): 243-248.
Schwartz, Mark, W, Hoeksema, JasonD, Gehring, Catherine, A, Johnson, Nancy C, Klironomos, John,N, Abbott Lynette, and Pringle, Anne. “The promise and the potential consequences of the global transport of mycorrhizal fungal inoculum.” Ecology Letters. 9 (2006): 501-515.
Sinegani, Sinegani, Ali, Akbar., and Yeganeh, Masomeh, Elyasi. “The occurrence of arbuscular mycorrhizal fungi in soil and root of medicinal plants in Bu-Ali Sina garden in Hamadan, Iran.” Biological Journal of Microorganism. 5.20(2017):43-59.
Soka, Georey and Ritchie, Mark, E. “Arbuscular mycorrhizal symbiosis, ecosystem processes and environmental changes in tropical soils.” Applied Ecology and Environmental Research.13.1 (2014):229-245.
Smith S.E. and Zhu, Y.G. “Application of arbuscular mycorrhizal fungi: Potentials and challenges.” In: Bio-Exploitation of Filamentous Fungi, edited by BP Stephen & KD Hyde, Fungal Diversity Research Series.6 (2001) :291-308.
Smith, S.E., and Read, D.J., (2008). Mycorrhizal Symbiosis. London, Academic press.
Srivastava R.B., Kumar, Vimal., Prasad, Chandan., Deka D.C., and Singh, Lokendra. “Oroxylum indicum- a medicinal plant of North East India: An overview of its nutritional, remedial, and prophylactic properties.” Journal of Applied Pharmaceutical Science 3.1(2013): 104-112.
Sundar S.K., Palavesam A., Parthipan B. “AM Fungal Diversity in Selected Medicinal Plants of Kanyakumari District, Tamil Nadu, India.” Indian Journal of Microbiology. 51.3(2011):259-265.
Tamilarasi, S, Nanthakumar, K, Karthikeyan, K, Lakshmanaperumalsamy, P.” Diversity of root associated microorganisms of selected medicinal plants and influence of rhizomicroorganisms on the antimicrobial property of Coriandrum sativum.” Journal of Environmental Biology. 29(2008):127-134.
Zhao, Ke, Penttinen, Petri., Chen, Qiang., Guan Tongwei, Lindstrom, Kristina., Ao, Xiaoling., Zhang, Lili and Zhang, Xiaoping. “The rhizospheres of traditional medicinal plants in Panxi, China, host a diverse selection of actinobacteria with antimicrobial properties.” Applied Microbiology and Biotechnology .94(2012):1321-1335.
Zubek, Szymon., Błaszkowski, Jansuz., Seidler-Łoz˙ykowska, Katarzyna., Baba, Wojciech., Mleczko, Piotr. “Arbuscular mycorrhizal fungi abundance, species richness and composition under the monocultures of five medicinal plants.” Acta Scientiarum Polonorum Hortorum Cultus 12 (2013): 127-141.
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